• You can press   in the menu to switch to the combine tab; this saves time and makes it easy to see which items you can currently create
• Psychostimulants are very useful, use them once an area is cleared to scavenge any leftover items
• Enemies cannot follow you everywhere, there are certain boundaries to their movement, like doors to different areas; use this to your advantage
• X-Ray glasses are cool, especially on Madhouse. Carry them if you can spare the item slot. 

• Speaking of X-Ray glasses: Below you will find a list of all the unlockables in Resident Evil 7:

You will get this trophy as soon as you finish watching the first few cutscenes of the game, right after you exit your car. 

At the start of the game, you see Mia recording a video for Ethan were she confesses that she lied to him. Here only message is that he needs to stay away and should not come look for her. However, in the next cutscene you see Ethan driving towards a mansion located in Dulvey, Louisiana while having a phone call with an unknown person. He claims that Mia has contacted him to come and get her, while he thought she was dead, because he hadn't heard of her for over 3 years.

You will get this trophy as soon as you beat the first boss of the game and escape from the guest house.

Once you reach the Attic in the guest house, you will have to face Mia one last time. If you try to climb up the ladder, Mia will be waiting for you with a chainsaw. A difficult fight will follow, as your ammo is very limited and the area is really small. Once you have defeated her, go into the storage room attached to the attic where an unpleasant surprise will be waiting for you. Here you will meet Jack Baker for the first time as he knocks you out from behind and welcomes you to the family.

You will be awarded this trophy upon successfully obtaining three key items which will open the front door of the main house.

The three items in question are three dog heads. The first can be found in the Living Room, right next to the room you came to after being taken from the guest house. Use the Clock Pendulum you find in the Main Hall with the Grandfather Clock in the Living Room and the White Dog's Head is yours. The Blue Dog's Head can be found in the Recreation Room upstairs. It's hidden inside a book. For the last Dog's Head you need to head down into the Processing Area. At the end of this section, you will meet Jack Baker for yet another challenging boss fight. There is a pillar in the middle of the room and there are a couple of corpses hanging from the ceiling. You can kick those corpses with   to slow Jack down and make it easier to attack him. Use your shotgun to shoot him in the face until he goes for the huge chainsaw. After this, grab the smaller chainsaw for yourself and start your new career as an experimental hairdresser. Attack him until he goes down, then use  and  to plunge your saw deep into his brains. Do this twice (on normal) and you should be able to take him apart for now. Head back upstairs and use the last head in the front door to unlock it. Leave the main house and the trophy will unlock.

This trophy will unlock once you have successfully retrieved a certain key item from the Old House.

You will be awarded this trophy after a major boss fight. You might have escaped the Baker family home, yet the nightmare is far from over...

You will be awarded this trophy after unveiling the truth behind the fate of the Baker family and escaping the ship.

This ending is linked to a specific story decision you make when you are two thirds through the game.

After fighting mutant Jack Baker you will be faced with two damsels in distress but you will have only one shot of serum left. Choose to save Mia for Ending 1.

This ending is linked to a specific story decision you make when you are two thirds through the game.

In order to unlock this trophy, you need to complete the game on Easy difficulty. All difficulty-related trophies in this game are stackable, which means you will unlock this trophy if you beat the game on a harder difficulty as well. For more information about this trophy, see the  Who's Your Daddy Now? trophy.

In order to unlock this trophy, you need to complete the game on Normal difficulty. All difficulty-related trophies in this game are stackable, which means you will unlock this trophy if you beat the game on a harder difficulty as well. For more information about this trophy, see the  Who's Your Daddy Now? trophy.

There are a lot of minor and major differences when playing on Madhouse difficulty. First of all, the enemies will hit you harder and take more damage. They also spawn in different locations, so watch out for fresh jump scares. Saves will also be limited, since you'll need cassette tapes in order to save. There is no shortage of those tapes though, and they can be found in plain sight next to the cassette recorders. The game does still make an auto save for you in a few spots, but they are very limited as well. Below is an overview off all the auto save locations on Madhouse difficulty. Be sure to keep them in mind while you are considering to make a manual save or not.

1. Just before you lose your hand to Mia's chainsaw attack in the Guest House.
2. After you are brought to the Main House and break out of the chair in the room where you are having dinner with the Baker family.
3. After watching Lucas' video in the Testing Facility where the TV explodes.
4. Right before you have to fight Jack for the third time on the boat yard, after taking the serum from Zoe.
5. Right after the boat ride, where you get to play as Mia.
6. When you reach Floor 1 of the Wrecked Ship, right after the cutscene where Evie runs off and says 'You said we'd be a family. YOU SAID!'.
7. Right after starting the videotape sequence on the Wrecked Ship that reveals Mia's history as Eveline's caretaker.
8. After exiting the elevator on floor S2 in the Wrecked Ship, still during the video tape sequence.
9. Right before the only skippable cutscene in the game where the Wrecked Ship gets destroyed.
10. Right before the next cutscene in the game where Jack will reveal Eveline was behind all of this and that he nor his family are killers.
11. After you get to play as Ethan again, right after Mia saves you from the cocoon.
12. During the section in the attic where you will have to inject the serum into Eveline, where she tries to push you away with her force waves.
13. The last auto save of the game will be right after you've injected Eveline with the serum.

As you will notice, there is a massive gap between #2 and #3. Make use of your manual saves throughout this section wisely, as you most likely won't have too much trouble from that point on. There are plenty of cassette tapes in the game, and most of them are actually lying around in plain sight around the recorders. If you collect only those which are easy to find, you will be easily able to save about a dozen times. Another big difference on Madhouse difficulty has to do with the Antique Coins. There are 33 of them on Madhouse difficulty, while easy and normal had only 18, and they are placed differently. Getting them is not only worth it because of the trophy, they will also unlock juicy items which will make your life on Madhouse much easier.

Last but not least, the boss fights will also way harder than on easy or normal difficulty. However, once you have access to the first item box, things will lighten up considerably. A lot of bosses can be dispatched within minutes using the Circular Saw, same counts for most regular enemies. If you combine this with The Essence of Defence and The Secrets of Defence, beating the game on Madhouse should become manageable. If you are having difficulties with a specific boss fight, check out PowerPyx's boss fight strategies in the video below.

Resident Evil 7 Madhouse Difficulty Boss Strategy Video Guide

More often than not you will find yourself in a situation where you are feeling safer with the door being closed behind you. Most doors in the game will close automatically for you once you get out of range, however for this trophy you will have to manually close any door yourself. You can close an open door by pressing the  button on it.

Guarding is one of the more useful gameplay mechanics in the game and will ensure that you will only take a minimal amount of damage from an enemy attack. To guard an enemy attack, all you have to do is simply hold the  button and you will automatically block an incoming attack.

You will come across many opportunities in the game where you can earn this trophy. The first opportunity is at the very start of the game, right before entering the guest house. You will come across a burned-out fireplace in the open with a bag lying right next to it. Take the bag and examine it closely. You will find a driver's license inside of it. The trophy will unlock once you pick up the driver's license by pressing the   button.

Lock picks can be used to open desk drawers and item boxes for some additional goodies to help you on your journey through the Baker family nightmare. You will find your first lock pick in the first save room. Look out for a yellow box. Pick it up and examine it. You can open it by pressing   once you have found the correct angle. Use the lock pick on any applicable lock - for example the little table in the hallway near the Living Room - and the trophy will unlock. 

You can find a Shadow Plinth in a small alcove next to the main hall. Look out for a waist-high stone column illuminated by a spotlight. You are supposed to use a wooden statuette with it and align it so that its shadow matches the shape on the picture on the wall. For this trophy, however, simply try using a different object, for example the Clock Pendulum, the knife or one of the Dog’s Heads. The trophy will pop shortly after doing this.

Psychostimulants is a new item in the RE universe. Using it allows you to see the locations of all collectibles for a limited amount of time. Little markers will pop up on screen and you can even see them through walls, floors and ceilings. The nearer you get to an item, the bigger the marker will become.

The first opportunity to unlock this trophy is after you have solved the puzzle involving the Wooden Statuette in the main hall. Squeeze through the new passageway and you will find Psychostimulants in the room with the deer. The trophy will pop upon usage, regardless of whether you found any items using it or not. 

Once you have reached the Old House you will finally get the opportunity to increase your limited item slots. There is a backback sitting on a table and waiting for you in the save room in the northwest of the area. Simply pick it up to increase your item capacity and unlock this trophy. If you are having trouble finding it refer to the video below.

In the Bag Video Guide

The easiest method to obtain this trophy is killing the big flies in the Old House. They are roughly the size of a kitten so you can't really miss them. It takes only one slash with your trusty knife to kill them; once you do, the trophy will unlock. Keep in mind that the swarming flies do not count, you need to go for the bigger ones. 

You will encounter insect-covered doors in the Old House for the first time. You will notice that some of the doors (normal doors but also cabinet doors) are covered with some greyish stuff. Upon approaching those doors you will find out that you were not looking at mold, but at hundreds of tiny itsy-bitsy spiders, ready to jump at you! Eww! The normal (and most efficient) method of dealing with this problem would be to burn them with fire. However, for this trophy you'll want to use the knife and keep slashing until they are all gone. Don't worry, they don't deal a huge amount of damage and it doesn't take too long to get rid of them. Once the door is cleared the trophy is yours. 

This trophy probably will not come naturally since it requires you to waste a lot of ammunition on an enemy you do not have to fight. Once you have reached the Old House you will soon meet Marguerite Baker. Do not worry, the encounter is story-related and you cannot miss her. She will be wandering through the house and it is actually pretty easy to avoid her, since she is carrying a lamp which makes it easy to see her coming. For this trophy, however, you need to pump lead into her until she runs away. Make sure to build the Flamethrower before attempting this to make fighting off the insect swarms she sends at you easier. Otherwise just keep attacking her until she has had enough. As with all enemies, headshots do way more damage, so go for those. Once she pushes you to the ground to run away, the trophy is yours. 

This trophy can only be obtained during the Jack boss fight in the Processing Area. Once you have dealt enough damage, Jack will have enough of you and go for a massive scissor-like double chainsaw. One of his attacks with this weapon is a charging attack. Jack will be coming at you with open scissors, trying to cut you in half. If successful, this can be a one hit kill, so be careful. To avoid the attack and get this trophy, simply press   at the right moment to duck. Jack will miss and the trophy will unlock. 

This trophy can easily be obtained in the Old House, were you will be introduced to Insect Swarms. Let one of those swarms get near you, then simply use your shotgun to blast right into it. Not the most efficient way to deal with insect swarms, but certainly the way to go to get this trophy. 

This trophy can only be obtained during the boss fight against Marguerite in the greenhouse. It is recommended to create a manual save file before going for this, in case you kill her before unlocking the trophy.

Start the fight and hit her a couple of times. Once you have dealt some damage to her, she will start climbing the ceilings and walls. Keep a close eye on her since she is about to jump at you. She will stop crawling and look at you before leaping off, so watch out for that. Use your shotgun and fire at her shortly after she jumped. You need to hit her in mid-air for the trophy to unlock. If you did not get the timing right, wait for her to climb the walls and try again. If you need some visual aids refer to the video below:

Fly Swatter Video Guide

Remote Bombs will become available on the ship during the later phase of the game. They are extremely powerful and allow for strategic enemy disposal. You can place them on the ground with   and detonate them with  . For this trophy, however, you need an enemy to grab you while having a remote bomb equipped. You will be prompted to attach the bomb to the enemy. The bomb will go off, saving your ass and unlocking another trophy. 

Chem Fluid - an item vaguely resembling an urine bag - and Strong Chem Fluid (same in red) can be used to create a number of new items, ranging from healing items to ammunition. To combine them, select the Fluid and choose 'Combine with' or simply press  while you are in the menu to switch to the combine tab. Below you can find a table presenting all possible combinations. Once you have successfully created at least one of each the trophy will unlock.

You will find the "Mia" videotape in the Recreation Room on the second floor of the Old Mansion. Conveniently enough there is a TV and a VCR in the same room, so you can go for this trophy right away.

You will be playing as Mia and you must not be spotted by Marguerite to unlock this trophy. Avoiding her is not terribly hard; simply hide every time you can hear her come nearer. You can use the boxes at the beginning of the area and wait until she has left through the closed door, this will give you some time to sneak through the area. If she returns you can hide outside, just around the corner. You will be able to see her rummaging around inside through the cracks in the wall, so you'll know when it is safe to go back inside again. If Marguerite should see you simply wait for her to grab you, this will trigger a checkpoint restart, allowing you to try again. Of course you can replay the video on any VCR in the game whenever you like. Below is a video guide if you prefer visual aids.

Can't Catch Me Video Guide

The "Happy Birthday" videotape can be found in the cabinet in the attic of the Old House, in the room where you obtain the Red Key Card. The good news is that you do not have to complete the tape within 5 minutes on your first attempt. It will remain in your inventory and you can watch it again and again on any VCR in the game (e.g. the one in the trailer). This also means that you do not have to use the telescope to find out the combination for the medicine cabinet, since it will not change. Simply follow the step-by-step instructions below or watch the video and you should not have any issues with the trophy requirements. 

1. Grab the Candle.

2. Go into the room with the Birthday Cake, this will trigger the actual "game".

3. Open the medicine cabinet (combination Chainsaw Man, Hat with Raven, Baby, found by getting Telescope out of Toilet and cleaning it via Cake room entrance, then using it on monitor wall)

4. Get the Winding Key from the keg

5. Leave the room and re-light the candle using the stove.

6. Burn the Straw Doll using the stove to get the Dummy Finger

7. Burn the rope on the door with the candle.

8. Grab the Balloon from the next room

9. Use the Balloon with the leaky pipe to get the Quill Pen

10. Use the Dummy Finger and the Quill Pen with the left hand of the guy who had the candle, then use the Winding Key in his torso to activate him

11. Enter the word Loser in the lock of the door in the room with the balloons. 

12. Grab the Valve.

13. Use the Valve with the water pipe in the cake room.

14. Re-light the Candle on the stove.

15. Put the Candle on the cake.

Out Before Dessert Video Guide

In order to unlock this trophy, you need to watch all the 4 videotapes in a single playthrough. Videotapes are one of the 4 types of collectables in the game. For a detailed overview of every collectable in the game, see the  Mr. Nowhere trophy.

In order to unlock this trophy, you need to obtain all of the 18 antique coins in Easy or Normal difficulty. Antique coins are one of the 4 types of collectables in the game. The reason you have to collect these coins on Easy or Normal difficulty, is because the Madhouse difficulty has its own different set of collectable antique coins. For a detailed overview of every collectable in the game, see the  Mr. Nowhere trophy.

There are 33 Antique Coins on Madhouse difficulty, while easy and normal had only 18, and they are placed differently. Getting them is not only worth it because of the trophy, they will also unlock juicy items which will make your life on Madhouse much easier. PowerPyx compiled a very useful video guide which will allow you to catch them all:

Mad Pelicans Video Guide

In order to unlock this trophy, you need read all of the 32 files in a single playthrough. Files are one of the 4 types of collectables in the game. For a detailed overview of every collectable in the game, see the  Mr. Nowhere trophy.

In order to unlock this trophy, you need to destroy a Mr. Everywhere statuette. Mr. Everywhere statuettes are one of the 4 types of collectables in the game. For a detailed overview of every collectable in the game, see the  Mr. Nowhere trophy.

There are 4 different type of collectables in the game: Files, Antique Coins, Mr. Everywhere statuettes, and Videotapes. Below is an overview of all the collectables sorted in the order you should get them:

Resident Evil 7 - Mr. Nowhere Trophy Guide

Outside the Guest House

1 Files | 0 Antique Coins | 0 Mr. Everywhere statuettes | 0 Videotapes

Guest House

2 Files | 1 Antique Coins | 0 Mr. Everywhere statuettes | 1 Videotapes

#	Type	Map Location	Description
2	Videotape #1 Derelict House Footage	Guest House 1F	Once you've entered the Guest House, go upstairs and you will find yourself the videotape lying on a desk, right next to the tape recorder.
3	Antique Coin #1	Guest House 1F	Easily Missable! To get the first antique coin of the game, you will need to open a drawer with a lockpick in the kitchen of the Guest House. The drawer can only be opened while playing the first videotape. The lockpick will be hidden underneath you right as you start the videotape. Simply walk back a bit and look down to pick it up. Then make your way to the kitchen and open the drawer. Once you are back from playing the videotape, go back to the kitchen and the drawer will be opened. The antique coin is inside of it.
4	File #2 List of Names (Front)	Guest House B1	Once you progress through the Guest House and make your way down to some creepy underground basement, you will eventually come across Mia lying on a bed behind a locked cell door. You will need pick up the bolt cutters in the next room to get it open. Right next to the bolt cutters lies the file. In order to get two separate files, you will have to read both sides of the file, meaning you will have to press the  button on both the front and back side of the file.
5	File #3 List of Names (Back)	Guest House B1	See File #2.

Old Mansion

10 Files | 6 Antique Coins | 6 Mr. Everywhere statuettes | 1 Videotapes

Yard

1 Files | 2 Antique Coins | 1 Mr. Everywhere statuettes | 0 Videotapes

Old House

3 Files | 3 Antique Coins | 4 Mr. Everywhere statuettes | 0 Videotapes

Yard #2

1 Files | 0 Antique Coins | 0 Mr. Everywhere statuettes | 0 Videotapes

Old Mansion #2

3 Files | 0 Antique Coins | 1 Mr. Everywhere statuettes | 1 Videotapes

Barn

1 Files | 2 Antique Coins | 1 Mr. Everywhere statuettes | 0 Videotapes

Boat House

0 Files | 0 Antique Coins | 1 Mr. Everywhere statuettes | 0 Videotapes

Wrecked Ship

5 Files | 4 Antique Coins | 2 Mr. Everywhere statuettes | 1 Videotapes

#	Type	Map Location	Description
55	Antique Coin #15	Wrecked Ship S1	After the flashback with the two crew members the only way to continue will be trough the hatch in the floor. Crawl through the tunnel until you come to a T-junction. Turn right and grab the coin at the end of the dead end before moving on.
56	Antique Coin #16	Wrecked Ship S1	After climbing a rather long ladder from 2S to 1S, you will have do drop down into a narrow room filled ankle-deep with water. The coin is in the water, slightly left of the place you landed on. Make sure to grab this one, since it is easily missable!
57	File #23 Jim's Letter	Wrecked Ship 1F	After coming up the set of stairs and meeting Evie (You said!) take a right and follow the hallway. Take another right at the end and then go through the first door on the left. You'll be in the bunk room, the letter can be found on the bunk bed in the left corner.
58	File #24 Giovanni's will	Wrecked Ship 1F	Exit the bunk room (see collectible above) through the door in the south and you will find this document on a little table in the next room.
59	Videotape #4 Old Videotape	Wrecked Ship 3F	This collectible is unmissable, since you need to watch it to progress in the story. Provided you have collected the other three videotapes in the same playthrough,  Be Kind, Please Rewind will unlock after putting the tape into the VCR.
60	File #25 Orders	Wrecked Ship 2F	After gaining control of Mia in the videotape section, turn left and check the desk with the oil lamp for this note.
61	Mr. Everywhere #16	Wrecked Ship 4F	Welcome to the bridge! Search for a set of stairs in the south-west of the area. The stairs are blocked by some canisters, but if you listen carefully you will hear the familiar ticking of a Mr Everywhere. Check the railing on the left to find a Mr. Everywhere hiding behind it.
62	File #26 Mechanic's Memo	Wrecked Ship 1F	Use the lug wrench form the Captain's cabin to open the hatch in the elevator. Jump into the broken elevator which is stuck between 1F and 2F and you will find this note on its wall.
63	Mr. Everywhere #17	Wrecked Ship 2F	Climb out of the elevator (2F) and turn left. Follow the hallway to the very end, go into the little room at the end (north-east on the map) and climb up the ladder. Once you are up there turn around and shoot the Mr. Everywhere on the opposite side.
64	File #27 Tattered Secret Document	Wrecked Ship 3F	Go back to the elevator and make your way to 3F. Climb out of it and you will find a sample kit with a file attached to it in the corner to your left.
65	Antique Coin #17	Wrecked Ship 3F	Proceed through the door next to the sample kit and you will find a toolbox in the next room. Open the lock of the toolbox with a lock pick and you will find an antique coin inside of it.
66	Antique Coin #18	Wrecked Ship S2	This collectible requires you have corrosive. Be sure to have at least 1 corrosive substance left in your inventory. If you are 1 short, you might've missed the one in the save room on 2F. After you restore power to the elevator by finding the power cable and fuse, you will go to S2. Here you will have to walk through some passages avoiding quite a few zombies. Eventually right after you've come across a Fat Molded, you will go down some stairs and come across a locked box where you will have to use the corrosive on. Open the box and you will find the antique coin inside of it. After you pick up this collectible, you should unlock the  Pelicans in Your Pocket trophy.

Swamp

0 Files | 0 Antique Coins | 1 Mr. Everywhere statuettes | 0 Videotapes

Salt Mine

5 Files | 0 Antique Coins | 2 Mr. Everywhere statuettes | 0 Videotapes

#	Type	Map Location	Description
68	Mr. Everywhere #19	Salt Mine	When you get to the salt mine, you have to investigate the hideout. At some point, you will have to push a mine cart in order to clear the way. In the next room, you will have to climb up some stairs to continue your way. Instead, walk past the stairs and the mine cart, all the way to the back end of the area. There is a statuette hidden pretty well on the platform above you in front of the barrels. You will have to shoot it in order to destroy it.
69	File #28 Email Log	Salt Mine	Continue your way by going up the stairs and proceeding through the door on the corridor. To your left you will see a workbench with a laptop on it. Press the  on the laptop to get access to the email log.
70	File #29 Infection Report	Salt Mine	Proceed to the next room and you will get to a room where you will have to create another serum. As soon as you get in this room, you will see a report lying on the shelve right in front of you.
71	File #30 E-Nectrotoxin Document	Salt Mine	In the middle of the room you will come across a necrotoxin container. Open it and read the note attached to it.
72	File #31 R&D; Report 1/2	Salt Mine	On the workbench behind the necrotoxin container, you will see another report lying around for you to pick up.
73	File #32 R&D; Report 2/2	Salt Mine	Turn around and go up the small elevation. Here you will find desk with lots of books and folder lying on top of it. Here you will also find the last file you need to collect in order to get the  The Devil Is in the Details trophy.
74	Mr. Everywhere #20	Salt Mine	The last statuette, and also the very last collectible of the game, will be at the very end of the salt mine, right after getting back to the Main House where you started at the beginning of the game. Walk past the grandmother's wheelchair and take a look at the bookcase to your right before leaving the passage. You will find your last Mr. Everywhere statuette standing on one of the bottom shelves. Destroy it to unlock the  Mr. Nowhere trophy.

Four hours is a very generous time frame for a Resident Evil 7 speedrun, so it is recommended to combine this trophy with  Resource Manager - don't open the item box more than three times - and  Walk it Off - only use three First Aid Meds or less.

A speedrun time of about 2 - 3 hours is perfectly possible on your second playthrough, especially if you are referring to a video walkthrough whenever you are lost. Use  to pause the timer while doing this! Dying or restarting checkpoints manually will add to your time, so it is recommended to create multiple manual save files you can fall back to if the shit hits the fan and you have to replay a certain section. To check your time, press   and go to Stats. Here are some reference times to help you evaluate your progress. The times are very lenient, but as you can see from the final time you could even be a tad slower and it would still be in the trophy time frame.  

Below is a video walkthrough which combines the speedrun trophy with  Resource Manager and  Walk it Off.

Just Get Me Outta Here, Resource Manager and Walk it Off Video Guide

The most important point about this trophy is that the game forces you to use the Item Box three times! This means you are not allowed to use it at all apart from those occasions if you want to obtain this trophy. There is a workaround for the third time you are forced to use the box, so technically it might be okay to use it once in between. However, it's actually not necessary to do that since this trophy is not that hard at all.

First off, you should obviously be playing on easy when attempting this. As stated above, it's sensible to combine this trophy with  Just Get Me Outta Here and  Walk it Off. Since you will be throwing away most of your healing items you shouldn't have too many issues with inventory space. If you were following the roadmap you shouldn't be forced to pick up and Coins, either. The tree times you are forced to use the Item Box are before and after the 'Birthday Game' and after escaping from the Old Ship.

If you die during certain boss battles, you will be presented with a 'Manage Items' option. This does not count towards this trophy, so technically you could skip the Item Box after the Old Ship and get your weapons through this option. However, this should only be necessary if you somehow managed to open an Item Box by accident. If you want to check on that, hit   and select stats. 

Refer to  Just Get Me Outta Here Here for a video walkthrough for this trophy. 

This trophy is way easier than it sounds. It can (and obviously should) be done on easy and can be combined with  Just Get Me Outta Here and  Resource Manager. The good news is that you can actually 'walk damage off', you will regenerate a small section of health, so you won't be at the brink of death forever after screwing up once. You are allowed to heal three times, so you can save those for boss fights if you like. It is recommended not to pick up healing items or to throw them away so you won't accidentally use them. If you want to check on that, hit    and select stats.  Steroids don't count and can be used; they will heal you completely and increase your total health permanently, giving you some additional healing chances.

Refer to  Just Get Me Outta Here for a video walkthrough for this trophy.